The Precision Biosystems BlotCycler improves western blot results in several ways, mainly by addressing the limitations of the traditional manual process:
1. Accuracy and reproducibility:
- Manual western blots involve multiple steps with solution changes and incubations, making it prone to human error in volume measurement, timing, and handling. The BlotCycler automates these steps, precisely dispensing solutions and controlling incubation times, leading to highly reproducible results.
2. Consistency:
- BlotCycler eliminates variations in blotting due to factors like rocking or shaking inconsistencies in manual protocols. Its controlled agitation ensures even antibody distribution and blotting uniformity across the membrane.
3. Reduced hands-on time and labor:
- Automating the entire blotting process frees up researchers for other tasks, boosting lab productivity. Both the BlotCycler Mini and BlotCycler Touch offer different tray configurations that are easily interchanged.
4. Antibody saving:
- The BlotCycler’s recirculation system minimizes antibody waste by collecting and reusing unused antibody solutions. This can significantly reduce antibody costs, especially for expensive ones.
5. Improved data quality:
- Consistent and accurate blotting leads to cleaner blots with lower background noise, enabling better signal detection and quantification.
Overall, the BlotCycler offers several advantages over manual western blotting, leading to more accurate, reproducible, and cost-effective results. This makes it a valuable tool for researchers who want to improve the quality and efficiency of their western blot experiments.
