We recommend you wear gloves and use forceps for reproducible western blot assays Follow these tips for improving the reproducibility of your western blot assay, and for achieving experimental results that are actually publishable. Each antibody-antigen interaction has unique characteristics. The interaction of antisera with protein epitopes in western blotting depend upon a [...]
Bad Western blots. It happens! Use our Western blot troubleshooting guide to help you sort it out so that your next blot is your best blot.
Emerge from the darkness – see BlotCycler™ automated western blot processor at the NIH Research Festival.
Scientists seeking reproducible data in their experiments are often confounded with the results from western blot assays. The steps for processing western blots require precise performance of blocking and antibody incubation. When these steps are performed manually, any error or variation that occurs is directly responsible for inconsistent results (Koller and Wätzig, 2005, Precision [...]
In 2009, several people in Mangala village, located in the Democratic Republic of Congo, Africa, contracted acute hemorrhagic fever, and a novel rhabdovirus, Bas-Congo Virus or BASV, was detected in one of the serum samples. The precise mode of transmission remained unclear, but health officials were worried that BASV was emerging as a human [...]
No need to sit and watch your western blot to obtain precise results. If you are like most scientists studying proteins, you are processing western blots manually, so you know the steps required for incubation. First, you add the blocking agent, wait a specified amount of time. Next, add the primary antibody and [...]
When the World Anti Doping Agency (WADA) needed an automated Western blot device to test samples from over 10,500 of athletes, they chose the BlotCycler™ for fast, reliable, reproducible results with SAR-PAGE.
When the WADA accredited laboratory in Lausanne, Switzerland needed to analyze over 1000 samples for the 2014 FIFA World Cup, they used the BlotCycler™ for speed of processing and reliable results for their Western blots!
When researchers investigated the optimization of SAR-PAGE, they used the BlotCycler™ for incubation and washing to increase the reproducibility of results and economize labor time and increase signal-to-noise ratio.
Research Application Note: The BlotCycler™ Automated Western Blot Processor In Investigation of Rapamycin Treatment and Cardiac Functions
When researchers investigating Rapamycin treatment of healthy pigs and cardiac functions, they used the BlotCycler™ Automated Western Blot Processor for increased reliability and validity.
When researchers evaluated the potential use of clone 9G8A for doping control purposes, they used the BlotCycler™ automated Western blot processor to enhance reliability and validity of their findings.