Improved Western Blot Processing is now at your fingertips by using the BlotCycler™
Rockland Immunochemicals and Precision Biosystems used fluorescent labeled secondary antibodies to compare manual and automated processing. The multi-color visualization presented on the poster demonstrates that BlotCycler™ is able to produce clear and high quality results, allowing a straightforward process for the identification of individual protein.
Using two identical protocols, we showed that the BlotCycler™ demonstrates improved Western Blot Processing. It increases the signal to noise ratio by reducing background and increasing signal intensity. The BlotCycler™ provides extremely good reproducibility that is probably related to minimal variability in washing and incubation times. BlotCycler™ can be used to optimize the concentrations of primary and secondary antibodies potentially providing huge saving by decreasing the amount of antibody used.
Thus the BlotCycler™ offers a time-saving alternative to manual processing of fluorescent western blot by providing very low background staining, high signal-to-noise ratio, ability to multiplex detection and excellent reproducibility. BlotCycler™ provides opportunity for easy optimization of western blotting and significantly improves the quality of the procedure.