Why Run Western Blots at 4 degrees?

There are a few reasons why running western blots at 4 degrees is important.

First, it helps to prevent the proteins from denaturing. Denaturation is when the proteins lose their shape and function. This can happen if the proteins are exposed to heat or other harsh conditions. Running the western blot at 4 degrees helps to keep the proteins in their native state, which is necessary for accurate results.

Second, running the western blot at 4 degrees helps to reduce the background noise. Background noise is caused by proteins that are not of interest. When the western blot is run at 4 degrees, the background noise is reduced, which makes it easier to see the proteins of interest.

Third, running the western blot at 4 degrees helps to improve the resolution of the results. Resolution is the ability to distinguish between two closely spaced bands. When the western blot is run at 4 degrees, the resolution is improved, which makes it easier to see the bands of interest.

Overall, running western blots at 4 degrees is important for several reasons. It helps to prevent the proteins from denaturing, reduce the background noise, and improve the resolution of the results.