The BlotCycler Mini or Touch by Precision Biosystems offers several advantages over manual western blotting methods by minimizing human error, ensuring consistency, improving efficiency, and potentially saving on expensive reagents. This leads to more accurate, reproducible, and cost-effective results, making it a valuable tool for researchers aiming to improve the quality and efficiency of their [...]
Western blotting is a powerful technique, but it's also susceptible to errors that can compromise your results. Automating as many steps as possible will reduce the chance of error and improve overall results. The following are suggestion that can reduce your chance of error: Sample preparation: Protease inhibitors: Include protease inhibitors in your lysis buffer to [...]
The Precision Biosystems BlotCycler improves western blot results in several ways, mainly by addressing the limitations of the traditional manual process: 1. Accuracy and reproducibility: Manual western blots involve multiple steps with solution changes and incubations, making it prone to human error in volume measurement, timing, and handling. The BlotCycler automates these steps, precisely dispensing [...]
Ever wish you could perform the blocking and incubation protocols precisely every time? Would you like to make the Western Blotting Process Easy and reduce common errors? The following benefits are easily achieved using the BlotCycler Touch or BlotCycler Mini: Eliminate variations in fluid delivery and timing! Get reproducible results, repeatedly! Perform membrane processing precisely, [...]
Antibody validation is the process of ensuring that an antibody specifically recognizes and binds to its intended target molecule, with minimal non-specific binding. It's critical for guaranteeing the reliability and reproducibility of research results, especially in downstream applications like immunohistochemistry, western blotting, and ELISA. There are a number of validation parameters to consider, and the [...]
The selection of the primary antibody is crucial for the success of a western blot experiment. The primary antibody is responsible for specifically binding to the protein of interest, which allows it to be visualized on the blot. There are several factors that must be considered when selecting a primary antibody: Specificity: The primary antibody needs [...]
Western blotting is a very reliable method to measure protein quantity and provide protein identification using antibodies and molecular weight (MW). Other methods for protein quantification, such as Bradford assays and UV-Vis spectroscopy, are not as specific or sensitive as Western blotting. They can also be affected by the presence of other substances in the sample, [...]
The BlotCycler Touch is a versatile and powerful blotting processing system that can be used for a variety of applications, including antibody titration. The BlotCycler Touch has the capability to run six different primary and secondary antibodies simultaneously. Due to this unique feature, six different concentrations of primary antibodies can be tested to determine the [...]
Western blotting is a standard laboratory tool for detecting and quantifying specific proteins in a sample. Due to the number of steps involved, it is important to be aware of the common errors that can occur, which can lead to misleading results. The following are typically the most common errors in Western blotting protocol: Incorrect [...]
Several studies have shown that washing at 4 degrees Celsius can improve the specific signal for western blotting. For example, one study found that washing at 4 degrees Celsius increased the specific signal by up to 50%. https://precisionbiosystems.com/wp-content/uploads/2019/08/mayo-app-note-blotcycler.pdf Another study found that washing at 4 degrees Celsius reduced the background signal by up to 80%. Washing [...]
