Each antibody-antigen interaction has unique characteristics. Interaction of antisera with protein epitopes in western blotting depends upon a number of factors, all contributing to the final signal/noise ratio. Knowledge and control of those factors allows for modifications and optimization of the procedure. Prepare the membrane For size-related identification of an interaction between protein and antibody [...]
In 2009, several people in Mangala village, located in the Democratic Republic of Congo, Africa, contracted acute hemorrhagic fever, and a novel rhabdovirus, Bas-Congo Virus or BASV, was detected in one of the serum samples. The precise mode of transmission remained unclear, but health officials were worried that BASV was emerging as a human [...]
Discussion of research comparing manual vs. Western blot automation. The automated Western blot increased signal to noise ratio and signal intensity.