We recommend you wear gloves and use forceps for reproducible western blot assays Follow these tips for improving the reproducibility of your western blot assay, and for achieving experimental results that are actually publishable. Each antibody-antigen interaction has unique characteristics. The interaction of antisera with protein epitopes in western blotting depend upon a [...]
Discussion of research comparing manual vs. Western blot automation. The automated Western blot increased signal to noise ratio and signal intensity.