reproducibility of Western blot

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Scientists have a love/hate relationship with western blotting.

2019-11-18T07:11:44-05:00November 16th, 2019|Application, Reproducible immunoblot|

Routinely used to study cancer, HIV, sports doping, Lyme disease, mad cow disease, etc., etc., western blots are notoriously unreliable. Variations in technique confound reproducible results. Retraction Watch, a blog that reports on retraction of scientific papers, questions whether western blots are trustworthy at all. What’s going on? Koller et al. writes that variable results [...]

General tips for western blot reproducibility

2019-10-07T11:24:52-04:00September 12th, 2019|Reproducible immunoblot, Western blot troubleshooting|

We recommend you wear gloves and use forceps for reproducible western blot assays Follow these tips for improving the reproducibility of your western blot assay, and for achieving experimental results that are actually publishable. Each antibody-antigen interaction has unique characteristics. The interaction of antisera with protein epitopes in western blotting depend upon a [...]