Routinely used to study cancer, HIV, sports doping, Lyme disease, mad cow disease, etc., etc., western blots are notoriously unreliable. Variations in technique confound reproducible results. Retraction Watch, a blog that reports on retraction of scientific papers, questions whether western blots are trustworthy at all. What’s going on? Koller et al. writes that variable results [...]
We recommend you wear gloves and use forceps for reproducible western blot assays Follow these tips for improving the reproducibility of your western blot assay, and for achieving experimental results that are actually publishable. Each antibody-antigen interaction has unique characteristics. The interaction of antisera with protein epitopes in western blotting depend upon a [...]
Discussion of research comparing manual vs. Western blot automation. The automated Western blot increased signal to noise ratio and signal intensity.