Each antibody-antigen interaction has unique characteristics. Interaction of antisera with protein epitopes in western blotting depends upon a number of factors, all contributing to the final signal/noise ratio. Knowledge and control of those factors allows for modifications and optimization of the procedure. Prepare the membrane For size-related identification of an interaction between protein and antibody [...]
Bad Western blots. It happens! Use our Western blot troubleshooting guide to help you sort it out so that your next blot is your best blot.
Don't see the protein expression by Western blotting even after trying multiple antibodies and other protocol optimizations? The reason could be as simple as the protein loss from the membrane. Use our PVDF western blot troubleshooting strategy for greater success.